Mutukula is located in extreme southern Kyotera District at the international border between Uganda and Tanzania. The town is approximately 81 kilometers (50 mi), by road, south-west of Masaka, and the largest city in the sub-region. This location is approximately 211 kilometers (131 mi), by road, south-west of Kampala, the capital and largest city of Uganda. The coordinates of the town are 1°00’00.0″S, 31°25’00.0″E (Latitude:-1.0000; Longitude:31.4167).
The town of Mutukula is a border town between Uganda and Tanzania. At an altitude of 1,190 meters (3,900 ft), above sea level, Mutukula is an important border post and major crossing point, for both human and commercial traffic. In February 2016, the border post between both countries merged operations on both sides. Vehicles and passengers clear customs and immigration once in the country they are leaving, with officials from both countries present.
In 2017, the border crossing between Mutukula, Uganda and Mutukula, Tanzania was converted into a one-stop-border-post (OSBP), open 24 hours every day of the year. Vehicles, passengers, luggage, merchandise and pedestrians are cleared once by customs and immigration staff from both countries, at a station in the country of entry. This cuts the time it takes to cross by anywhere from 30 percent to 50 percent. Other stakeholders report reduction in crossing times by as much as 90 percent. As of July 2009, the population of Mutukula was estimated at 15,000 people. The following additional points of interest lie within the town limits or close to the edges of the town: (a) offices of Mutukula Town Council (b) Mutukula Airport, a military and civilian airport, located approximately 10 kilometers (6 mi) north of town (c) Mutukula prison, a medium security corrections facility (d) Mutukula central market (e) Mutukula One Stop Border Post.